Alkaline phosphatase (AP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides and proteins under alkaline conditions. The Dual-Color Alkaline Phosphatase Staining Kit is a histochemical assay for use on cells grown in tissue culture wells or dishes. The AP-positive, undifferentiated stem cells stain red or blue. The stain develops quickly, does not readily fade, and can be analyzed 15 to 30 minutes after application to the cultured cells. For added convenience, the stained samples can be kept at 4°C for up to one week for analysis.
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