The CUTANA™ CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from cells to PCR amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. Positive (H3K27me3) and negative (IgG) control antibodies are paired with the SNAP-CUTANA™ K-MetStat Panel of nucleosome spike-in controls to continuously monitor workflows and guide troubleshooting.
The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs.
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