Fiber-seq is a breakthrough approach that simultaneously maps genetic and epigenetic features from individual chromatin fibers. This single assay delivers insights that would normally require integrating data from multiple methods such as ATAC-seq, ChIP-seq and bisulfite sequencing. This kit includes reagents to go from cells to purified genomic DNA that can be used directly in either Pacific Biosciences® (PacBio® HiFi Sequencing) or Oxford Nanopore Technologies® (ONT® Nanopore Sequencing) library preparation workflows. The recommended input for Fiber-seq is 1,000,000 native human nuclei per reaction. The Fiber-seq Kit has been successfully applied to cryopreserved and lightly cross-linked nuclei.
• Consolidate multiple assays into ONE workflow (ATAC-seq, WGBS, chromatin protein mapping)
• Antibody-free protein footprinting at nucleotide resolution
• Seamless integration with existing long-read sequencing workflows
• Resolve complex, hard-to-map regions missed using short reads
• Powered by Hia5, the highest activity 6mA-methyltransferase available
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