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CUTANA Hia5 for Fiber-seq

CUTANA™ Hia5 is the key enzyme for Fiber-seq, a multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation and genetic variation at single-molecule resolution. By layering chromatin accessibility on top of standard LRS workflows, Fiber-seq uniquely links epigenetic information (DNA methylation) and complex genetic architecture (e.g., structural variants and haplotype phasing) to chromatin states within the same molecules of DNA.

Hia5 is a DNA adenine methyltransferase (MTase) that uses S-adenosylmethionine (SAM) to catalyze methylation at the N6 position of adenine, producing N6-methyladenine (6mA). Fiber-seq leverages the nonspecific activity of Hia5 to methylate adenines within accessible chromatin regions in situ. This records chromatin accessibility information onto native DNA while preserving endogenous methylation (5mC). Hia5-modified DNA is fully compatible with LRS on both Pacific Biosciences® (PacBio® HiFi Sequencing) and Oxford Nanopore Technologies® (ONT® Nanopore Sequencing) platforms.

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