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MVP Human Total RNA

Application- ready RNA. The quality of the RNA is assessed visually by observing distinct 28S and 18S ribosomal bands on a non-denaturing gel. Purity of the RNA is assessed by spectrophotometry (A260/A280 > 1.8). RNA is further shown to be free of contaminating RNases. Total RNA and Poly(A)+ RNA products are subjected to stringent quality assurance functional testing, including Northern analysis and RT-PCR. Products were isolated using a modified guanidinium thiocyanate method. Human tissue was obtained using protocols approved by an Institutional Review Board. Total RNA is enriched for mRNA by two successive rounds of Oligo(dT)-cellulose selection to produce our Poly(A)+ RNA. Well-documented donor and tissue pathology information

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