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Nucleosome, Recombinant Human, H3K4,9,14,18ac/H4K5,8,12,16ac dNuc, Biotinylated

EpiCypher

Catalog Code: 16-0374Product Unit: 50µg
POA

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3*; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains acetyl-lysine at positions 4, 9, 14, and 18 and H4 (created by a proprietary semi-synthetic method) contains acetyl-lysine at positions 5, 8, 12, and 16. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers, is useful for nucleosome assembly and contains a 5�� biotin-TEG group. *H3K4,9,14,18ac has a Cys to Ala substitution at position 110.

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