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PrecisionX Gene Knock-out HR Targeting Vectors

For HR-based applications needing the disruption of a particular protein-coding or non-coding gene of interest, a gene-knockout targeting vector would be desirable. This vector would include homology arms (0.5kb-1kb in length) at the 5' and 3' regions immediately flanking the DNA sequence of interest (e.g. exonic sequence) to specifically direct the targeting vector to insert an expression cassette where the targeted sequence lies, leading to the 'knock-out' of the sequence by gene addition. SBI's gene knockout targeting vectors (Cat #HR110PA-1, #HR210PA-1, #HR410PA-1, and #HR510PA-1) are designed to be vectors of choice for this application.

SBI also offers a series of three HR targeting vectors (Catalog #s HR700PA-1, HR710PA-1, and HR720PA-1) containing a PGK-hsvTK selection cassette that is located outside of the 3' MCS homology arm cloning site

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Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Puro-pA-MCS2]
Catalog Code: HR110PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]
Catalog Code: HR210PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]
Catalog Code: HR410PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2]
Catalog Code: HR510PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration
Catalog Code: HR700PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration
Catalog Code: HR710PA-1
Product Unit: 10µg POA

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration
Catalog Code: HR720PA-1
Product Unit: 10µg POA

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