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System Biosciences
Used to tag an endogenous sequence of interest with a marker to create a fusion or to co-express a marker tag with the endogenous sequence for various applications (e.g. tracking spatial dynamics of a protein, lineage tracking, etc.) Any type of DNA sequence, from protein-coding ORFs to non-coding sequences can be efficiently tagged or co-expressed with GFP and/or luciferase using these vectors. For protein-coding genes, the 3' end (typically the last exon) can be targeted using these vectors for efficient generation of C-terminal fusion tagged proteins or co-expression of the gene and the tag via T2A or IRES elements.
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