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AAVanced Cloning and Expression Vectors

rAAV expression vectors based on the commonly used AAV2 serotype. The expression vectors contain inverted terminal repeat (ITR) sequences at both ends of the DNA strand and room for an open reading frame encoding a transgene driven by an exogenous promoter. To produce a high titer of viral particles, expression and packaging vectors are transiently co-transfected into suitable mammalian virus producer cells (e.g. HEK 293T cells) for subsequent isolation of rAAV virus particles in culture media.

There is a limit for the size of the insert(s) that can be cloned into the vectors for efficient packaging into viral particles. The size limit of insert(s) that can be cloned should be less than 5kb (entire size of the cassette between the ITRs including insert) or packaging efficiency may be impacted.

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pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector
Catalog Code: AAV502A-1
Product Unit: 10µg POA

pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector
Catalog Code: AAV526A-1
Product Unit: 10µg POA

pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector
Catalog Code: AAV527A-1
Product Unit: 10µg POA

pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector
Catalog Code: AAV528A-1
Product Unit: 10µg POA

pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector
Catalog Code: AAV503A-1
Product Unit: 10µg POA

pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector
Catalog Code: AAV536A-1
Product Unit: 10µg POA

pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector
Catalog Code: AAV537A-1
Product Unit: 10µg POA

pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector
Catalog Code: AAV538A-1
Product Unit: 10µg POA

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