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Dual Media Set (EB + OB)

Catalog Code: M3011Product Unit: 1 set
$151.00 Excl. GST

Contains two specially formulated media: Expansion Broth (EB) and Overexpression Broth (OB). For expansion, E.coli cells are grown in EB which keeps the production of recombinant protein repressed. To initiate high level protein expression, OB is simply added to the culture. By using the Dual Media Set, protein overexpression can be reliably controlled for many recombinant proteins. In some circumstances, when the expressed protein is either toxic or insoluble, overexpression may be counter-productive. In such cases, protein production can be kept at a minimum by adding the inducer IPTG (for lac-based promoters) to cells growing in EB.

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