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Mononucleosomes, Recombinant, Hemi-methylated 199x601 DNA, Non-biotinylated

EpiCypher

Catalog Code: 16-2143Product Unit: 50µg
POA

Mononucleosomes assembled from recombinant histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P06897; H2B-P02281; H3-Q92133; H4-P62799) wrapped by 199 base pairs of DNA containing the 601 positioning sequence DNA. The the 199bp DNA sequence contains a centrally positioned 601 sequence, identified by Lowary and Widom, which is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly. The 601 sequence is flanked by a hemi-methylated 26 bp sequence as shown in application notes.

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