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Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated

Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains monomethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.* H3.2 K4me1 has a C110A mutation

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Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated
Catalog Code: 16-0321-50
Product Unit: 50µg POA

Nucleosome, Recombinant Human, H3K4me1 dNuc, Biotinylated
Catalog Code: 16-0321
Product Unit: 20µg POA

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