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pBluescript II RI Predigested Vector

Catalog Code: 212250Product Unit: 55 reactions
POA

The pBluescript II RI predigested vector is a high-copy-number pUC-based plasmid with ampicillin resistance and the convenience of blue-white color screening. The vector supplied is predigested with EcoR I restriction enzyme and then is CIAP-treated to reduce the background of nonrecombinants. The multiple cloning site (MCS) contains unique restriction enzyme recognition sites organized with alternating 5 ́- and 3 ́- overhangs to allow serial exonuclease III/mung bean nuclease deletions. T3 and T7 RNA polymerase promoters flank the polylinkers for in vitro RNA synthesis. The choice of promoter used to initiate transcription determines which strand of the DNA insert will be transcribed. BssH II sites flank the T3 and T7 promoters. This rare six-base restriction enzyme allows the insert plus the RNA promoters to be excised and used for gene mapping.

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