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SNAP-ChIP OncoStat Panel

A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243*; H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The mononucleosomes constitute a pool of 1 unmodified plus 7 histone H3.3 point mutations: H3.3K4M, H3.3K9M, H3.3K27M, H3.3G34R, H3.3G34V, H3.3G34W, H3.3K36M. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ( barcode�) at the 3' end that can be deciphered by qPCR or next-generation sequencing. Each of the 8 nucleosomes in the pool is wrapped by 2 DNA species, each containing a distinct barcode (侫� and B�, see SNAP-ChIP Manual) allowing for an internal technical replicate.

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SNAP-ChIP OncoStat Panel
Catalog Code: 19-2100
Product Unit: 200µL POA

SNAP-ChIP OncoStat Panel
Catalog Code: 19-2001
Product Unit: 20µL POA

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