Allows for a simple and streamlined approach to ChIP and improved ChIP DNA purification. Protocol involves covalent cross-linking of protein-DNA complexes with formaldehyde followed by cell lysis and chromatin shearing. A ChIP-grade antibody (user supplied) is used with Protein A magnetic beads to immunoprecipitate the protein-DNA complexes of interest. Following reverse crosslinking, RNase A and Proteinase K treatments, the DNA is eluted in a minimal volume of buffer (≥6µL) using a unique micro-elution spin column, eliminating the need for messy precipitations.
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